2'-Thiol Modified Oligonucleotides
A reporter molecule or reactive group can be spaced at varying distances (through tethers of varying lengths) from a probe oligonucleotide. In structure-probing techniques it is crucial to have tethers that function as "molecular rulers" in order to determine all feasible possibilities for the docking of two or more domains (Earnshaw et al., J. Mol. Biol., 1997, 274, 197). Libraries of space probes should be useful in assessing distances at the molecular level.
Our oligonucleotides possessing tethers with internal disulfide linkages can be used to cross-link between nucleotides and proteins and within the same oligonucleotide to assess distances.
FOR RESEARCH USE ONLY