Fimers and Non-Vanilla Custom Oligonucleotides
We are now pleased to offer custom synthesis of affordable, high-quality non-vanilla oligonucleotides. Our selection of currently available monomers can be incorporated either at the 5'- or 3'-terminal ends and internally within the oligo in a variety of scales, concentrations, and purification levels. Our proprietary MOX/SUC chemistries assure maximum versatility in the design and overall physical properties of the linkers and spacers attached to the oligonucleotides. The oligonucleotides can be individually tailored to meet your requirements in terms of length, flexibility, hydrophobicity, charge density and multiplicity of the tethers. A full range of modifications and possible applications for custom oligonucleotides is outlined below.
Fimers are primers with proprietary chemical modifications that inhibit unwanted reactions during cycle sequencing (e.g., primer-dimer artifacts and non-specific PCR). more >
Amino-modified oligonucleotides have been routinely employed in solid support and label (or functionality) attachment chemistries. The 5'-terminus of the oligonucleotide is normally the target end for modification because of the ease of incorporation as the last step in automated synthesis. more >
We also offer 2'-amino modified oligonucleotides through the use of our SUC/MOX precursor strategy. By using this methodology we can incorporate amino-modifiers internally (currently at pyrimidine nucleosides - C and U analogs) within the oligonucleotide. more >
Biotin is widely used for DNA/RNA detection/isolation due to the extremely high affinity of the biotin-streptavidin interaction (association constant 1015/M). Biotin moieties can be incorporated within the oligo at any position and multiple times if desired. We have long and super-long tethering arms for improved binding kinetics, increased binding capacity of large DNA fragments, and for accessibility to enzymatic events occurring at the solid-phase surface. more >
Oligonucleotides having a high number of covalently attached functional groups can easily be synthesized using our MOX/SUC precursor chemistries. more >
Pteridines are naturally occurring, highly fluorescent compounds that are structurally similar to purines and that were first isolated from butterfly wings in 1889. The pteridines developed by Hawkins and co-workers are incorporated into DNA through a deoxyribose moiety identical to that of native DNA with no "linker-arm" attachment involved. more >
A disulfide thiol modifier is available for 5'-end modification. A variety of tether lengths holding the thiol moiety can be varied to space the oligonucleotide away at a defined distance. more >
We also offer 2'- thiol modified oligonucleotides. A reporter molecule or reactive group can be spaced at varying distances (through tethers of varying lengths) from a probe oligonucleotide. In structure-probing techniques it is crucial to have tethers that function as "molecular rulers" in order to determine all feasible possibilities for the docking of two or more domains. Libraries of space probes should be useful in assessing distances at the molecular level. more >
Complex probes can be made to suit your assay design. A combination of two or more different fluorescent dyes and other reporter groups can be incorporated on the oligonucleotide.
· Custom RNA
High purity RNA can be synthesized using standard monomers with established 2'-O-tBDMS protecting groups. Custom RNA has successfully been used in antisense and RNA interference technologies. Furthermore, we can modify the RNA at the 5'- or 3'-ends and within the oligo at the 2'-position of C and U if desired.
· Phosphorothioates, Methyl Phosphonates, etc.
We can synthesize oligonucleotides used for antisense and antigene applications.
FOR RESEARCH USE ONLY