Real Time Detection of DNA Probe Digestion
ORDERING INFO

Item Cat. No Units
6-MAP 6MAP nucleotide

(2001) Anal. Biochem 298 231

(2001) CBB 34 257 (review)

(1998) SPIE 3256 68

(1997) Anal. Biochem 244 86

(1997) Biophys J 73 3277

(1995) NAR 23 2872


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The rate of P1 nuclease cleavage of 6MAP from the oligonucleotide demonstrates how this reaction can be observed in real time.

Emission Fluorescence of P1 nuclease digestion of 6-MAP containing oligo. The blank - no P1 added.

6MAP

Nucleoside analog Adenosine
Excitation maximum 330 nm
Emission maximum 435 nm
Quantum yield 0.39
Availability Custom oligonucleotide synthesis

FOR RESEARCH USE ONLY
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