Biotinylated Oligonucleotides


    Biotin is widely used for DNA/RNA detection/isolation due to the extremely high affinity of the biotin-streptavidin interaction (association constant 1015/M). Biotin moieties can be incorporated within an oligo on any place and in any number. We have long and super-long tethering arms covalently attached to biotin for improved binding kinetics, increased binding capacity of large DNA fragments, and for accessibility to enzymatic events occurring at the solid-phase surface.

    The size of the DNA oligonucleotide covalently attached to biotin seems to limit the binding capacity to immobilized streptavidin (Sabanayagam et al., Nucleic Acids Res., 2000, 28, e33). We have discovered that in the use of biotinylated primers for sequencing reactions, the ability to capture large DNA Sanger fragment pools with paramagnetic streptavidin beads was directly related to the length of linker arms holding the biotin moiety. The capturing efficiency for these long DNA fragments was maximized when using our "super-long" tethering arms with biotin. We speculate that steric interference of the DNA to the surface-bound streptavidin is minimized by increasing the length of the tethering arm (NNNA 2003, Oligonucleotide Synthesis 2004).

Other applications:




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